Cell Signaling Technology

Product Pathways - Development

Snail (L70G2) Mouse mAb #3895

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 29 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Snail (L70G2) Mouse mAb detects endogenous levels of total Snail protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human Snail protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT-116, NIH/3T3 and Rat2 cells using Snail (L70G2) Mouse mAb.

Background

Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10).

  1. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-3161.
  2. Cano, A. et al. (2000) Nat. Cell Biol. 2, 76-83.
  3. Batlle, E. et al. (2000) Nat. Cell Biol. 2, 84-89.
  4. Yokoyama, K. et al. (2003) Int. J. Oncol. 22, 891-898.
  5. Yokoyama, K. et al. (2001) Oral Oncol. 37, 65-71.
  6. Vega, S. et al. (2004) Genes Dev. 18, 1131-1143.
  7. Zhou, B.P. et al. (2004) Nat. Cell Biol. 6, 931-940.
  8. Bachelder, R.E. et al. (2005) J. Cell Biol. 168, 29-33.
  9. Yook, J.I. et al. (2005) J. Biol. Chem. 280, 11740-11748.
  10. Yang, Z. et al. (2005) Cancer Res. 65, 3179-3184.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products