Product Pathways - Wnt / Hedgehog / Notch
Snail (L70G2) Mouse mAb #3895
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 29 | Mouse IgG1kappa |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Snail (L70G2) Mouse mAb detects endogenous levels of total Snail protein.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a recombinant human Snail protein.
Background
Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10).
- Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-3161.
- Cano, A. et al. (2000) Nat. Cell Biol. 2, 76-83.
- Batlle, E. et al. (2000) Nat. Cell Biol. 2, 84-89.
- Yokoyama, K. et al. (2003) Int. J. Oncol. 22, 891-898.
- Yokoyama, K. et al. (2001) Oral Oncol. 37, 65-71.
- Vega, S. et al. (2004) Genes Dev. 18, 1131-1143.
- Zhou, B.P. et al. (2004) Nat. Cell Biol. 6, 931-940.
- Bachelder, R.E. et al. (2005) J. Cell Biol. 168, 29-33.
- Yook, J.I. et al. (2005) J. Biol. Chem. 280, 11740-11748.
- Yang, Z. et al. (2005) Cancer Res. 65, 3179-3184.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.