Cell Signaling Technology

Product Pathways - Translational Control

PARN (P620) Antibody #3899

Applications Reactivity Sensitivity MW (kDa) Source
W H (B) (Dg) Endogenous 78 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  B=Bovine  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PARN (P620) Antibody detects endogenous levels of total PARN protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences surrounding Pro620 of human PARN. Antibodies are purified by Protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PARN (P620) Antibody.

Background

Cellular levels of mRNAs are controlled by mRNA stability, the rate of synthesis and the rate of degradation. The presence and length of the poly(A) tail has been associated with mRNA stability (1). Exonucleolytic shortening of the poly(A) tail is the process that initiates the decay of many eukaryotic mRNAs (2). Poly(A)-specific ribonuclease (PARN) is the enzyme responsible for initiation of deadenylation and exonucleolytic shortening of mRNA transcripts. Through an evolutionarily conserved mechanism, PARN also translationally silences selective mRNAs during early embryonic development (3). PARN is constitutively expressed in most mammalian tissues and plays a critical role in the post-transcriptional control of gene expression (4).

  1. Morales, J. et al. (1997) J Biol Chem 272, 6607-13.
  2. Wilson, T. and Treisman, R. (1988) Nature 336, 396-9.
  3. Sachs, A.B. et al. (1997) Cell 89, 831-8.
  4. Fritz, D.T. et al. (2004) Cell Biochem Biophys 41, 265-78.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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