Product Pathways - Complementary Reagents
Rabbit (DA1E) mAb IgG XP® Isotype Control #3900
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IHC-P IF-IC F ChIP | Endogenous | Rabbit IgG |
Applications Key:
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
ChIP=Chromatin IP
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Source / Purification
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (right).
Flow Cytometry
Flow cytometric analysis of K-562 cells, untreated (green) or STI-treated (blue), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).
Flow Cytometry
Flow cytometric analysis of HeLa cells using PTEN (138G6) Rabbit mAb #9559 Antibody (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).
IF-IC
Confocal immunofluorescent analysis of A549 cells using β-Tubulin (9F3) Rabbit mAb #2128 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of normal rat cerebellum using DARPP-32 (19A3) Rabbit mAb #2306 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-P
Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Chromatin IP
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The relative abundance of each DNA sequence enriched by Rabbit (DA1E) mAb IgG XP® Isotype Control (red) is compared to the amount of the same DNA sequence enriched by the histone H3-specific immunoprecipitations (blue).
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
- 4340 Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
- 3452 Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
