Product Pathways - Companion Products
Rabbit (DA1E) mAb IgG XP® Isotype Control #3900
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IP IHC-P IF-IC F ChIP | Endogenous | Rabbit IgG |
Applications Key:
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
ChIP=Chromatin IP
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Source / Purification
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (right).
Flow Cytometry
Flow cytometric analysis of K-562 cells, untreated (green) or STI-treated (blue), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).
IF-IC
Confocal immunofluorescent analysis of A549 cells using β-Tubulin (9F3) Rabbit mAb #2128 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of normal rat cerebellum using DARPP-32 (19A3) Rabbit mAb #2306 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-P
Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Chromatin IP
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The relative abundance of each DNA sequence enriched by Rabbit (DA1E) mAb IgG XP® Isotype Control (red) is compared to the amount of the same DNA sequence enriched by the histone H3-specific immunoprecipitations (blue).
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
- Zhang, S.J. et al. (2011) Chin Med J (Engl) 124, 3118-26. Applications: Flow Cytometry
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Companion Products
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
- 4340 Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
- 3452 Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
