Product Pathways - Development
AFP (3H8) Mouse mAb #3903
|3903S||100 µl (10 western blots)||---||In Stock||---|
|3903||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
AFP (3H8) Mouse mAb detects endogenous levels of total AFP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human AFP.
Flow cytometric analysis of HepG2 cells using AFP (3H8) Mouse mAb (blue) compared to Mouse (G3A1) mAb IgG1 Isotype Control #5415.
Confocal immunofluorescent analysis of HepG2 cells using AFP Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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