Product Pathways - Stem Cell and Lineage Markers
UTF1 Antibody #3909
|W||M R (H)||Endogenous||40||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
UTF1 Antibody detects endogenous levels of total UTF1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide correponding to amino acid sequence near the C-terminus of human UTF1. Antibodies are purified by Protein A and peptide affinity chromatography.
Undifferentiated embryonic cell transcription factor 1 (UTF1) is expressed in cells of the inner cell mass and the epiblast (1). Expression is down-regulated with development, although it is maintained in the embryonic germ cells and in the adult gonads (1). Reduced expression in embryonic stem cells (ESCs) is associated with failure to differentiate properly, although self-renewal is unaffected (2). UTF1 is tightly associated with chromatin in mouse and human ESCs and may be involved in maintaining an epigenetic environment necessary for the pluripotent state (2,3). Co-expression of UTF1 with reprogramming factors c-Myc, Oct-4, Sox2 and KLF4, along with siRNA knock-down of p53 increased efficiency of induced pluripotent stem cell generation by 100 fold (4).
- Okuda, A. et al. (1998) EMBO J 17, 2019-32.
- van den Boom, V. et al. (2007) J Cell Biol 178, 913-24.
- Kooistra, S.M. et al. (2009) Stem Cell Res 2, 211-8.
- Zhao, Y. et al. (2008) Cell Stem Cell 3, 475-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.