Product Pathways - Stem Cell and Lineage Markers
UTF1 Antibody #3909
PhosphoSitePlus® protein, site, and accession data: UTF1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M R (H) | Endogenous | 40 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3909:
- Western Blotting
Specificity / Sensitivity
UTF1 Antibody detects endogenous levels of total UTF1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide correponding to amino acid sequence near the C-terminus of human UTF1. Antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from rat testes, mouse embryo E6.5 and E8.5, mouse brain and rat brain and using UTF1 Antibody.
Background
Undifferentiated embryonic cell transcription factor 1 (UTF1) is expressed in cells of the inner cell mass and the epiblast (1). Expression is down-regulated with development, although it is maintained in the embryonic germ cells and in the adult gonads (1). Reduced expression in embryonic stem cells (ESCs) is associated with failure to differentiate properly, although self-renewal is unaffected (2). UTF1 is tightly associated with chromatin in mouse and human ESCs and may be involved in maintaining an epigenetic environment necessary for the pluripotent state (2,3). Co-expression of UTF1 with reprogramming factors c-Myc, Oct-4, Sox2 and KLF4, along with siRNA knock-down of p53 increased efficiency of induced pluripotent stem cell generation by 100 fold (4).
- Okuda, A. et al. (1998) EMBO J 17, 2019-32.
- van den Boom, V. et al. (2007) J Cell Biol 178, 913-24.
- Kooistra, S.M. et al. (2009) Stem Cell Res 2, 211-8.
- Zhao, Y. et al. (2008) Cell Stem Cell 3, 475-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
