Product Pathways - Tyrosine Kinase / Adaptors
p56Dok-2 Antibody #3914
|W||H (M)||Transfected Only||56 to 58||Rabbit|
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
p56Dok-2 Antibody detects transfected levels of total p56Dok-2 proteins. The antibody does not cross-react with other p62Dok family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues at the carboxy-terminal sequence of human p56Dok-2. The antibodies are purified by protein A and peptide affinity chromatography.
Docking proteins are substrates of tyrosine kinases that function in the recruitment and assembly of specific signal transduction molecules. There are five members in the p62dok family, p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1-3), characterized by the presence of an amino-terminal PH domain, a central PTB domain and numerous potential sites of tyrosine phosphorylation. Tyrosine phosphorylation of p56Dok-2 occurs upon stimulation of cells with a variety of stimuli, or in cells transformed by oncogenic tyrosine kinases such as v-Src and Bcr-Abl (3-5). Based on the presence of several signaling domains (PH, PTB domain, tyrosine residue and proline-rich regions), it has been proposed that the p62dok family act as docking proteins that link RTKs to signal transduction pathways. p56Dok-2 has been proposed to be a negative regulator of cytokine-induced proliferation in T cells (5). Phosphorylated Tyr351 of p56Dok-2 mediates an association with the SH2 domain of Nck (4).
- Master, Z. et al. (2001) EMBO J. 20, 5919-5928.
- Grimm, J. et al. (2001) J. Cell. Biol. 154, 345-354.
- Cristofano, A. D. et al. (1998) J. Biol. Chem. 273, 4827-4830.
- Jones, N. and Dumont, D.J. (1999) Curr. Biol. 9, 1057-1060.
- Nemorin, J.G. and Duplay, P. (2000) J. Biol. Chem. 275, 14590-14597.
- Roumiantsev, S. et al. (2004) Distinct stem cell myeloproliferative/T lymphoma syndromes induced by ZNF198-FGFR1 and BCR-FGFR1 fusion genes from 8p11 translocations. Cancer Cell 5, 287-298. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.