Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

DDR1 Antibody #3917

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 125 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DDR1 Antibody detects endogenous levels of total DDR1 protein. The antibody does not cross-react with other proteins of the DDR family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly598 of human DDR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from T-47D cells using DDR1 Antibody.

Background

The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1).

  1. Vogel, W. (1999) FASEB J. 13 Suppl, S77-S82.
  2. Ferri, N. et al. (2004) Am. J. Pathol. 164, 1575-1585.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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