Cell Signaling Technology

Product Pathways - Protein Folding

Bag1 (3.10G3E2) Mouse mAb #3920

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 52, 46, 33 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Bag1 (3.10G3E2) Mouse mAb detects endogenous levels of total Bag1 protein, including long, medium and short isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant full length human Bag1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bag1 (3.10G3E2) Mouse mAb.

Background

Bag1 belongs to the Bcl-2 associated athanogene (BAG) family of multifunctional proteins and was the first of six related proteins isolated from humans (1,2). This widely expressed protein interacts with a number of signaling molecules (including Bcl2, HGF receptor and Raf1) as it regulates signaling molecules in pathways involving cell survival, growth and differentiation. The most common role played by Bag1 protein is as an inhibitor of proteins favoring apoptosis (2-4). Bag1 also plays a role in Raf1 signaling and binds DNA as a transcription activator (4). Bag1 protein is a well-characterized inhibitor of its binding partner HSP70 (5). A conserved carboxy-terminal BAG domain within Bag1 interacts with the ATPase domain of HSP70 to negatively regulate heat shock protein chaperone activity (6,7). The multiple isoforms of Bag1 protein generated from a single transcript share a common ubiquitin homology domain and a carboxy-terminal Hsp70 binding region but differ in length and cellular localization. The 50 kDa long (Bag1-L) isoform also contains a nuclear localization signal and is often found in the nucleus where it activates transcription. The 46 kDa intermediate (Bag1-M) isoform is found mainly in the cytoplasm but can also translocate to the nucleus when associated with other proteins. The shorter 29-33 kDa isoforms (Bag1-S, Bag-1) isoforms are found primarily in the cytoplasm (8). High expression of the anti-apoptotic Bag1 protein correlates with increased survival in patients with particular forms of cancer, leading researchers to study possible therapeutic roles for Bag1 protein (9).

  1. Kudoh, M. et al. (2002) Cancer Res 62, 1904-9.
  2. Takayama, S. et al. (1995) Cell 80, 279-84.
  3. Bardelli, A. et al. (1996) EMBO J 15, 6205-12.
  4. Wang, H.G. et al. (1996) Proc Natl Acad Sci USA 93, 7063-8.
  5. Takayama, S. et al. (1997) EMBO J 16, 4887-96.
  6. Bimston, D. et al. (1998) EMBO J 17, 6871-8.
  7. Briknarová, K. et al. (2001) Nat Struct Biol 8, 349-52.
  8. Knee, D.A. et al. (2001) J Biol Chem 276, 12718-24.
  9. Millar, E.K. et al. (2009) Br J Cancer 100, 123-33.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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