Product Pathways - Protein Translation
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) #3929
|3929S||100 µl (10 western blots)||---||In Stock||---|
|3929||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, D. melanogaster||Endogenous||15 to 20||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: W=Western Blotting, F=Flow Cytometry
Specificity / Sensitivity
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1.
Western blot analysis of extracts from HeLa cells, serum-starved and stimulated with insulin (100 nM for 5 minutes), with (+) or without (-) λ phosphatase treatment, using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (upper) and 4E-BP1 (53H11) Rabbit mAb #9644 (lower).
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855.
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.