Product Pathways - PI3K / Akt Signaling
FoxO3a (75D8) Rabbit mAb (Biotinylated) #3938
|3938S||100 µl (10 western blots)||---||In Stock||---|
|3938||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||82 to 97||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: W=Western Blotting
Specificity / Sensitivity
FoxO3a (75D8) Rabbit mAb (Biotinylated) detects endogenous levels of total FoxO3a protein. The antibody does not cross-react with other members of the FoxO4 or FoxO1 families.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human FoxO3a.
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The unconjugated FoxO3a (75D8) Rabbit mAb #2497 reacts with human, mouse and rat Fox03a protein. CST expects that FoxO3a (75D8) Rabbit mAb (Biotinylated) will also recognize Fox03a in these species.
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).
- Anderson, M.J. et al. (1998) Genomics 47, 187-99.
- Galili, N. et al. (1993) Nat Genet 5, 230-5.
- Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
- Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
- Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
- Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
- Seoane, J. et al. (2004) Cell 117, 211-23.
- Arden, K.C. (2004) Mol Cell 14, 416-8.
- Yang, Y. et al. (2005) EMBO J 24, 1021-32.
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- 2497 FoxO3a (75D8) Rabbit mAb
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- 2486 Phospho-FoxO1 (Ser319) Antibody
- 9465 Phospho-FoxO3a (Ser318/321) Antibody
- 9471 Phospho-FoxO4 (Ser193) Antibody
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- 9472 FoxO4 Antibody
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.