Product Pathways - PI3K / Akt Signaling
FoxO3a (75D8) Rabbit mAb (Biotinylated) #3938
|W||H M R||Endogenous||82 to 97||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
FoxO3a (75D8) Rabbit mAb (Biotinylated) detects endogenous levels of total FoxO3a protein. The antibody does not cross-react with other members of the FoxO4 or FoxO1 families.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human FoxO3a.
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The unconjugated FoxO3a (75D8) Rabbit mAb #2497 reacts with human, mouse and rat Fox03a protein. CST expects that FoxO3a (75D8) Rabbit mAb (Biotinylated) will also recognize Fox03a in these species.
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).
- Anderson, M.J. et al. (1998) Genomics 47, 187-199.
- Galili, N. et al. (1993) Nat. Genet. 5, 230-235.
- Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
- Nakae, J. et al. (1999) J. Biol. Chem. 274, 15982-15985.
- Rena, G. et al. (1999) J. Biol. Chem. 274, 17179-17183.
- Guo, S. et al. (1999) J. Biol. Chem. 274, 17184-17192.
- Seoane, J. et al. (2004) Cell 117, 211-223.
- Arden, K.C. (2004) Mol. Cell 14, 416-418.
- Yang, Y. et al. (2005) EMBO J. 24, 1021-1032.
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- 2497 FoxO3a (75D8) Rabbit mAb
- 9464 Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody
- 9461 Phospho-FoxO1 (Ser256) Antibody
- 2486 Phospho-FoxO1 (Ser319) Antibody
- 9465 Phospho-FoxO3a (Ser318/321) Antibody
- 9471 Phospho-FoxO4 (Ser193) Antibody
- 2499 FoxO4 (55D4) Rabbit mAb
- 9472 FoxO4 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 3999 Streptavidin-HRP
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonal is produced under license (granting certain rights, including those under U.S. Patent Nos. 5,675,063 and 7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.