Cell Signaling Technology

Product Pathways - Translational Control

S6 Ribosomal Protein (54D2) Mouse mAb (Biotinylated) #3944

Applications Reactivity Sensitivity MW (kDa) Isotype
W F H M R Mk Endogenous 32 Mouse IgG1

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

S6 Ribosomal Protein (54D2) Mouse mAb (Biotinlyated) detects endogenous levels of total S6 ribosomal protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein.

Western Blotting

Western Blotting

Western Blot analysis of extracts from HeLa, NIH/3T3 and PC12 cells using S6 Ribosomal Protein (54D2) Mouse mAb (Biotinylated).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells, using S6 Ribosomal Protein (54D2) Mouse mAb (Biotinylated) (blue) compared to concentration matched Mouse mAb (MOPC-21) IgG1 Isotype control (Biotinylated) #4097 (red).

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested in-house by Western Blot and flow cytometric analysis. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (S6 Ribosomal Protein (54D2) Mouse mAb #2317).

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

  1. Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
  3. Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
  4. Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
  5. Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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