Product Pathways - Companion Products
Myc-Tag (71D10) Rabbit mAb (Biotinylated) #3946
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | All | Transfected Only | 65 | Rabbit IgG |
Applications Key:
W=Western Blotting
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 3946:
- Flow, Western Blotting
Specificity / Sensitivity
Myc-Tag (71D10) Rabbit mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Western Blotting
Western blot analysis of extracts from COS cells, untransfected or transfected with Myc-chorionic somatomammotropin hormone-like 1 (CSL), using Myc-Tag (71D10) Rabbit mAb (Biotinylated). Detection was performed with Streptavidin-HRP followed by LumiGlo® and Peroxidase #7003.
Flow Cytometry
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag (71D10) Rabbit mAb (Biotinylated).
Description
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with Myc tagged protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
- Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.
Application References
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Companion Products
- 3999 Streptavidin-HRP
- 2272 Myc-Tag Antibody
- 2278 Myc-Tag (71D10) Rabbit mAb
- 2279 Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2233 Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
