Cell Signaling Technology

Product Pathways - Apoptosis

Survivin (71G4B7) Rabbit mAb (Sepharose Bead Conjugate) #3947

Applications Reactivity Sensitivity MW (kDa) Isotype
IP H M R Endogenous 16 Rabbit IgG

Applications Key:  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Survivin (71G4B7) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total survivin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys60 of human survivin protein.

IP

IP

Immunoprecipitation of HeLa cell lysates using Survivin (71G4B7) Rabbit mAb (Sepharose Bead Conjugate) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423. The western blot was probed using Survivin (6E4) Mouse mAb #2802.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Survivin (71G4B7) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays.

Background

Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).

  1. Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
  2. Li, F. et al. (1998) Nature 396, 580-584.
  3. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
  4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
  5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
  6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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