Product Pathways - MAPK Signaling
Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) #3958
|3958S||100 µl (10 western blots)||---||In Stock||---|
|3958||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, D. melanogaster||Endogenous||45||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Chicken.
Specificity / Sensitivity
Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) detects endogenous levels of MEK1/2 only when phosphorylated at Ser217/221.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2.
Western blot analysis of extracts of HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Streptavidin-HRP (#3999).
Immunoprecipitation of extracts from HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Immobilized Streptavidin (Bead Conjugate) # 3419. Western blot analysis was performed using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154, Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 for detection.
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated antibody Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 reacts with human, mouse, rat, monkey and D. melanogaster phospho-MEK1/2 (Ser217/221). CST expects that Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) will also recognize phospho-MEK1/2 (Ser217/221) in these species.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
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- 9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
- 3999 Streptavidin-HRP
- 2338 Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
- 3419 Streptavidin (Sepharose Bead Conjugate)
- 9160 MEK1/2 HeLa Control Cell Extracts
- 9126 MEK1/2 (47E6) Rabbit mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
- 2352 MEK1 (61B12) Mouse mAb
- 9146 MEK1 (30C8) Rabbit mAb
- 9147 MEK2 (13E3) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.