Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) #3958

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Dm (C) Endogenous 45 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) detects endogenous levels of MEK1/2 only when phosphorylated at Ser217/221.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2.

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Streptavidin-HRP (#3999).

IP

IP

Immunoprecipitation of extracts from HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Immobilized Streptavidin (Bead Conjugate) # 3419. Western blot analysis was performed using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154, Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 for detection.

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated antibody Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 reacts with human, mouse, rat, monkey and D. melanogaster phospho-MEK1/2 (Ser217/221). CST expects that Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) will also recognize phospho-MEK1/2 (Ser217/221) in these species.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
  4. Cowley, S. et al. (1994) Cell 77, 841-852.

Application References

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Companion Products

Rabbit monoclonal antibody is produced Produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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