Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Nuclear Receptor Signaling

Nur77 (D63C5) XP® Rabbit mAb #3960

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H (Mk) Endogenous 70-80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nur77 (D63C5) XP® Rabbit mAb detects endogenous levels of total human Nur77 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu535 of human Nur77.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected (+) with a human Nur77 construct, using Nur77 (D63C5) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of of extracts from Jurkat cells, untreated (-) or treated with TPA #4174 (40 nM) and A23187 (2 μM), using Nur77 (D63C5) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with TPA #4174 and A23187 (blue), using Nur77 (D63C5) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat cells, untreated (left) or treated with TPA #4174 and A23187 (right), using Nur77 (D63C5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily (1-3). Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxy-terminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals (1-6). It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis (5,6). Nur77 binds to specific DNA elements leading to the regulation of target genes (7). As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL (8,9). Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization (4,10,11). Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis (12,13).

  1. Hazel, T.G. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 8444-8448.
  2. Chang, C. and Kokontis, J. (1988) Biochem. Biophys. Res. Commun. 155, 971-977.
  3. Milbrandt, J. (1988) Neuron 1, 183-188.
  4. Fahrner, T.J. et al. (1990) Mol. Cell. Biol. 10, 6454-6459.
  5. Liu, Z.G. et al. (1994) Nature 367, 281-284.
  6. Woronicz, J.D. et al. (1994) Nature 367, 277-281.
  7. Wilson, T.E. et al. (1991) Science 252, 1296-1300.
  8. Weih, F. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 5533-5538.
  9. Rajpal, A. et al. (2003) EMBO J. 22, 6526-6536.
  10. Hirata, Y. et al. (1993) J. Biol. Chem. 268, 24808-24812.
  11. Hazel, T.G. et al. (1991) Mol. Cell. Biol. 11, 3239-3246.
  12. Li, H. et al. (2000) Science 289, 1159-1164.
  13. Lin, B. et al. (2004) Cell 116, 527-540.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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