Product Pathways - Nuclear Receptor Signaling
Nur77 (D63C5) XP® Rabbit mAb #3960
|W IP IF-IC F||H (Mk)||Endogenous||70-80||Rabbit IgG|
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Nur77 (D63C5) XP® Rabbit mAb detects endogenous levels of total human Nur77 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu535 of human Nur77.
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected (+) with a human Nur77 construct, using Nur77 (D63C5) XP® Rabbit mAb.
Western blot analysis of of extracts from Jurkat cells, untreated (-) or treated with TPA #4174 (40 nM) and A23187 (2 μM), using Nur77 (D63C5) XP® Rabbit mAb.
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with TPA #4174 and A23187 (blue), using Nur77 (D63C5) XP® Rabbit mAb.
Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily (1-3). Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxy-terminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals (1-6). It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis (5,6). Nur77 binds to specific DNA elements leading to the regulation of target genes (7). As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL (8,9). Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization (4,10,11). Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis (12,13).
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For Research Use Only. Not For Use In Diagnostic Procedures.