Product Pathways - Adhesion
R-Ras Antibody #3962
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk B | Endogenous | 24 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
R-Ras Antibody detects endogenous levels of total R-Ras protein. The antibody does not cross-react with H-, K-, N- or M-Ras.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy-terminus of human R-Ras. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Hela, NIH/3T3, C6 and COS cells, using R-Ras Antibody.
IP
Immunoprecipitation of R-Ras from COS and C6 cell extracts followed by Western blot analysis, using R-Ras Antibody.
Background
R-Ras, a member of the small GTPase family, is homologous to H-, K- and N-Ras, but does not activate MAP kinase pathways and is only weakly oncogenic (1). Instead, R-Ras is engaged in integrin activation (2). The effector loop and the carboxy-terminal Pro-rich and prenylation sites of R-Ras are critical for integrin activation (3,4). Phosphorylation by EphB2 receptor tyrosine kinase and Src at Tyr66 of R-Ras suppresses integrin activity (5,6).
- Self, A. J. et al. (2001) J. Cell Sci. 114, 1357-1366.
- Zhang, Z. et al. (1996) Cell 85, 61-69.
- Oertli, B. et al. (2000) Oncogene 19, 4961-4969.
- Wang, B. et al. (2000) J. Biol. Chem. 275, 5222-5227.
- Zou, J. X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 13813-13818..
- Zou, J. X. et al. (2002) J. Biol. Chem. 277, 1824-1827.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
