Cell Signaling Technology

Product Pathways - Metabolism

GAPDH (14C10) Rabbit mAb (Alexa Fluor® 555 Conjugate) #3964

Applications Reactivity Sensitivity MW (kDa) Isotype
IF-IC H M R Mk Endogenous 37 Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GAPDH (14C10) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total GAPDH protein. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluroescent analysis of human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GAPDH (14C10) Rabbit mAb #2118.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

  1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
  2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
  3. Zheng, L. et al. (2003) Cell 114, 255-66.
  4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
  5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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