Cell Signaling Technology

Product Pathways - MAPK Signaling

Ras Antibody #3965

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Dm Pg Sc (Hm) (C) (X) (Z) (Dg) Endogenous 21 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Dm=D. melanogaster  X=Xenopus  Z=Zebrafish  Dg=Dog  Pg=Pig  Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras. This antibody may also cross-react with R-Ras and M-Ras.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues close to the amino-terminus of human K-Ras. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29, PAE, NIH-3T3, AR42J, PC12 and C6 cell lysates,using Ras Antibody.

Background

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

  1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-654.
  2. Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283.
  3. Buday, L. and Downward, J. (1993) Cell 73, 611-620.
  4. Huang, D.C. et al. (1993) Mol. Cell Biol. 13, 2420-2431.
  5. Bos, J.L. (1989) Cancer Res. 49, 4682-4689.
  6. Ma, J. and Karplus, M. (1997) J. Mol. Biol. 274, 114-131.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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