Product Pathways - MAPK Signaling
Ras Antibody #3965
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk Pg Sc Dm (C) (Hm) (X) (Z) (Dg) | Endogenous | 21 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
Sc=S. cerevisiae
C=Chicken
Hm=Hamster
Dm=D. melanogaster
X=Xenopus
Z=Zebra Fish
Dg=Dog
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras. This antibody may also cross-react with R-Ras and M-Ras.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues close to the amino-terminus of human K-Ras. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HT-29, PAE, NIH-3T3, AR42J, PC12 and C6 cell lysates,using Ras Antibody.
Background
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
- Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-654.
- Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283.
- Buday, L. and Downward, J. (1993) Cell 73, 611-620.
- Huang, D.C. et al. (1993) Mol. Cell Biol. 13, 2420-2431.
- Bos, J.L. (1989) Cancer Res. 49, 4682-4689.
- Ma, J. and Karplus, M. (1997) J. Mol. Biol. 274, 114-131.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3339 Ras (27H5) Rabbit mAb
- 3962 R-Ras Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
