Product Pathways - Chromatin Regulation / Epigenetics
TRRAP (D2966) Antibody #3966
|W||H M R Mk||Endogenous||434||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
TRRAP (D2966) Antibody detects endogenous levels of total TRRAP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human TRRAP protein. Antibodies are purified by peptide affinity chromatography.
Transformation/transcription domain-associated protein (TRRAP) is a highly conserved 434 kDa protein found in various multiprotein complexes, such as SAGA, PCAF, NuA4 and TIP60, which contain histone acetyltransferase (HAT) activity (1-4). TRRAP functions as an adaptor protein by binding directly to the transactivation domains of transcriptional activator proteins and facilitating the recruitment of HAT complexes to acetylate histone proteins and activate transcription (1-5). TRRAP is required for the transcriptional activation and cell transformation activities of c-Myc, E2F1, E2F4, p53 and the adenovirus E1A proteins (1,6,7). TRRAP is also essential in early development and is required at the mitotic checkpoint and for normal cell cycle progression (8,9). In addition, TRRAP has been shown to function in DNA repair. As part of the TIP60 complex, TRRAP is required for the acetylation of histone H4 at double-stranded DNA breaks and subsequent DNA repair by homologous recombination (10). In addition, TRRAP associates with the MRN (MRE11, RAD50, NBS1) complex, which lacks intrinsic HAT activity yet functions in the sensing and subsequent repair of double-stranded breaks by non-homologous DNA end-joining (11). TRRAP shows significant homology to the PI-3 kinase domain of the ATM family of kinases; however, amino acids that map to the catalytic site of the kinase domain are not conserved in TRRAP (1).
- McMahon, S.B. et al. (1998) Cell 94, 363-374.
- Grant, P.A. et al. (1998) Mol. Cell 2, 863-867.
- Ikura, T. et al. (2000) Cell 102, 463-473.
- Allard, S. et al. (1999) EMBO J. 18, 5108-5119.
- McMahon, S.B. et al. (2000) Mol. Cell. Biol. 20, 556-562.
- Deleu, L. et al. (2001) Oncogene 20, 8270-8275.
- Ard, P.G. et al. (2002) Mol. Cell. Biol. 22, 5650-5661.
- Herceg, Z. et al. (2001) Nat. Genet. 29, 206-211.
- Li, H. et al. (2004) EMBO J. 23, 4824-4834.
- Murr, R. et al. (2006) Nat. Cell Biol. 8, 91-99.
- Robert, F. et al. (2006) Mol. Cell. Biol. 26, 402-412.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3742 E2F-1 Antibody
- 9402 c-Myc Antibody
- 9281 Phospho-p53 (Ser392) Antibody
- 3427 Rad50 Antibody
- 4895 Mre11 Antibody
- 4847 Mre11 (31H4) Rabbit mAb
- 3001 Phospho-p95/NBS1 (Ser343) Antibody
- 3002 p95/NBS1 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.