Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

RAG1 (D36B3) Rabbit mAb #3968

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 119 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RAG1 (D36B3) Rabbit mAb detects endogenous levels of total RAG1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human RAG1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from MOLT-4 and HBP-ALL cells using RAG1 (D36B3) Rabbit mAb.

Background

The sequences encoding antigen receptors are split into multiple germline segments which are then combined by a process called V(D)J recombination during immune cells development. A variable (V) segment is combined with a joining (J) segment, and in some cases a D (Diversity) segment, to create the antigen-binding portion of the receptor. The recombined V(D)J segment is then spliced into exons that encode the constant region to produce mature mRNA (1,2). This essential process required for the development of functional immune T and B cells creates a vast diversity in these receptors (3,4). Initiation of this process follows binding of RAG1 (recombination activating gene 1) and RAG2 to the conserved recombination signal sequences (RSS) and the introduction of a double-strand break between the RSS and the coding sequence (5,6). RAG1 and RAG2 genes are located immediately adjacent to each other in the genome and lack introns in their coding regions in many species. RAG1 and RAG2 are coexpressed only in the B and T cell lineages and both are required for cleavage activity (7). RAG1 and RAG2 can also function as transposases, contributing to chromosomal translocations and lymphoid malignancy (8,9). Mutations in the RAG genes are associated with a spectrum of combined immune deficiencies in humans (10,11).

  1. Schatz, D.G. (2004) Semin Immunol 16, 245-56.
  2. Gellert, M. (2002) Annu Rev Biochem 71, 101-32.
  3. Alt, F.W. et al. (1992) Ann N Y Acad Sci 651, 277-94.
  4. Sadofsky, M.J. (2001) Nucleic Acids Res 29, 1399-409.
  5. Swanson, P.C. (2004) Immunol Rev 200, 90-114.
  6. Swanson, P.C. et al. (2009) Adv Exp Med Biol 650, 1-15.
  7. Fugmann, S.D. et al. (2000) Annu Rev Immunol 18, 495-527.
  8. Hiom, K. et al. (1998) Cell 94, 463-70.
  9. Agrawal, A. et al. (1998) Nature 394, 744-51.
  10. Villa, A. et al. (1999) J Clin Immunol 19, 87-97.
  11. Corneo, B. et al. (2000) J Biol Chem 275, 12672-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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