Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

GRB2 Antibody #3972

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Hm B Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster  B=Bovine
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

GRB2 Antibody detects endogenous levels of total GRB2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, A431, HEK293, Ramos, NIH/3T3, C6, COS, CHO and BAEC cells, using GRB2 Antibody.

Background

Growth factor receptor-binding protein 2 (GRB2) is a adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1 (1). The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of tyrosine 209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling (2).

  1. Pawson, T. and Scott, J.D. (1997) Science 278, 2075-2080.
  2. Li, S. et al. (2001) EMBO J. 20, 6793-6804.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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