Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

GRB2 Antibody #3972

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Hm Mk B Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GRB2 Antibody detects endogenous levels of total GRB2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, A431, HEK293, Ramos, NIH/3T3, C6, COS, CHO and BAEC cells, using GRB2 Antibody.

Background

Growth factor receptor-binding protein 2 (GRB2) is a adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1 (1). The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of tyrosine 209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling (2).

  1. Pawson, T. and Scott, J.D. (1997) Science 278, 2075-2080.
  2. Li, S. et al. (2001) EMBO J. 20, 6793-6804.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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