Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Gα(o) Antibody #3975

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Gα(o) Antibody detects endogenous levels of total Gα(o) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg15 of human Gα(o). Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Gα(o) Antibody.

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).

Gα(o) is the most abundant G protein in the brain and couples to serotonin, dopamine, GABA (B), opioid, glutamate and cholinergic receptors (4). Gα(o) -/- mice have neurological defects such as tremors, seizures and poor motor coordination (5).

  1. Offermanns, S. (2001) Oncogene 20, 1635-42.
  2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
  3. Umemori, H. et al. (1997) Science 276, 1878-81.
  4. Jiang, M. and Bajpayee, N.S. (2009) Neurosignals 17, 23-41.
  5. Jiang, M. et al. (1998) Proc Natl Acad Sci USA 95, 3269-74.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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