Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

TACE Antibody #3976

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 135 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

TACE Antibody detects endogenous levels of TACE protein. Additional bands result from differential glycosylation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Val200 of human TACE. Antibodies are purified by Protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts of various cell lines using TACE Antibody.

Background

TACE (TNF-α converting enzyme), also known as ADAM17, is a transmembrane metalloprotease that plays a key role in the cleavage of a number cell surface molecules in a process known as ¿shedding". TACE is abundantly expressed in many adult tissues, but in fetal development expression is differentially regulated (1). An important substrate of TACE is pro-TNF-α (1). Increased expression of TACE is associated with several pathological conditions including osteoarthritis and rheumatoid arthritis, where the pro-inflammatory effects of increased TNF-α contribute to disease pathogenesis (2,3). Regulation of other important molecules by TACE such as EGFR and Notch has recently been documented. TACE is responsible for the shedding of EGFR ligands such as amphiregulin and TNF-α. Some tumors have hyperactivated EGFR due to upregulated TNF-α production and upregulated TACE, making TACE a potential target for drug development (4). TACE activates Notch in a ligand-independent manner and has been shown to play a role in the development of the Drosophila nervous system (5). TACE has also been proposed to act as alpha-secretase for amyloid precursor protein (APP) (6), and to be involved in the renewal and proliferation of neural stem cells (7).

  1. Black, R.A. et al. (1997) Nature 385, 729-33.
  2. Amin, A.R. (1999) Osteoarthritis Cartilage 7, 392-4.
  3. Patel, I.R. et al. (1998) J Immunol 160, 4570-9.
  4. Kenny, P.A. (2007) Differentiation 75, 800-8.
  5. Delwig, A. and Rand, M.D. (2008) Cell Mol Life Sci 65, 2232-43.
  6. Deuss, M. et al. (2008) Curr Alzheimer Res 5, 187-201.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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