Product Pathways - Stem Cell Markers
Cdx2 Antibody #3977
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H | Endogenous | 38 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cdx2 Antibody detects endogenous levels of total Cdx2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the N-terminus of human Cdx2. Antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts of DLD-1 and Caco-2 cells using Cdx2 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon using Cdx2 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded DLD1 (left) or NTERA (right) cell pellets using Cdx2 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Cdx2 Antibody in the presence of control peptide (left) or antigen specific peptide (right).
Flow Cytometry
Flow cytometric analysis of DLD-1 cells, using Cdx2 Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of DLD-1 cells using Cdx2 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Cdx2, a homeobox domain-containing transcription factor, is a master regulator of the trophoectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development (1). Cdx2 is also involved in intestinal development (2), and gain of expression or loss of expression has been associated with various human malignancies such as Barret Esophagus (3) and colorectal cancer (4,5). Mouse embryonic stem cells deficient in Cdx2 display limited hematopoietic progenitor development and altered Hox gene expression (6), pointing to a role for Cdx2 in Hox gene regulation. Cdx2 is also implicated in the aberrant expression of Hox genes in human AML cell lines (7).
- Ralston, A. and Rossant, J. (2008) Dev Biol 313, 614-29.
- Traber, P.G. and Silberg, D.G. (1996) Annu Rev Physiol 58, 275-97.
- Shi, X.Y. et al. (2008) Am J Clin Pathol 129, 571-7.
- Bakaris, S. et al. (2008) Histol Histopathol 23, 1043-7.
- Guo, R.J. et al. (2004) Cancer Biol Ther 3, 593-601.
- Wang, Y. et al. (2008) Proc Natl Acad Sci U S A 105, 7756-61.
- Frohling, S. et al. (2007) Cell Cycle 6, 2241-5.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
