Cell Signaling Technology

Product Pathways - Lineage Markers

LIN28A (A177) Antibody #3978

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC F H M (Mk) Endogenous 26 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LIN28A (A177) Antibody detects endogenous levels of LIN28A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Ala177 of human LIN28A. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, including mouse embryonic stem cells (mESCs), using LIN28A (A177) Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NCCIT cells using LIN28A (A177) Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of P19 cells using LIN28A (A177) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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