Product Pathways - Stem Cell Markers
LIN28 (A177) Antibody #3978
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M (Mk) | Endogenous | 26 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
LIN28 (A177) Antibody detects endogenous levels of LIN28 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Ala177 of human LIN28. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, including mouse embryonic stem cells (mESCs), using LIN28 (A177) Antibody.
IHC-P (paraffin)
Imunohistochemical analysis of paraffin-embedded human lung carcinoma using LIN28 (A177) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse heart using Lin28 (A177) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using Lin28 (A177) Antibody.
Flow Cytometry
Flow cytometric analysis of NCCIT cells using LIN28 (A177) Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of P19 cells using LIN28 (A177) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
LIN28 is a conserved, developmentally regulated RNA binding protein (1). Overexpression of LIN28, in conjunction with Oct4, Sox2 and Nanog, has been shown to reprogram human fibroblasts to pluripotent, ES-like cells (2). LIN28 has recently been shown to be an inhibitor of the processing of microRNAs in embryonic stem and embryonic carcinoma cells (3,4). Let-7 is a microRNA that has been implicated in repression of oncogenes such as ras, myc and Hmga2 (5). In ES and EC cells, LIN28 blocks processing of the maturation of the let-7 family, but not other miRNAs. This selective inhibition occurs via LIN28 binding to terminal loop structures of let-7 miRNA precursors which may cause their sequestration in the cytoplasm, and away from the microprocessing complex in the nucleus (6).
- Balzer, E. and Moss, E.G. RNA Biol 4, 16-25.
- Yu, J. et al. (2007) Science 318, 1917-20.
- Newman, M.A. et al. (2008) RNA, Epub ahead of print.
- Viswanathan, S.R. et al. (2008) Science 320, 97-100.
- Cho, W.C. (2007) Mol Cancer 6, 60.
- Piskounova, E. et al. (2008) J Biol Chem, Epub ahead of print.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
