Product Pathways - Ca / cAMP / Lipid Signaling
Gα(o) Antibody (IP Preferred) #3981
|3981S||100 µl (10 western blots)||---||In Stock||---|
|3981||carrier free and custom formulation / quantity||email request|
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Applications Key: IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Human, Rat.
Specificity / Sensitivity
Gα(o) Antibody (IP Preferred) detects endogenous levels of total Gα(o) protein and is recommended for immunoprecipitation.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg15 of human Gα(o). Antibodies are purified by protein A and peptide affinity chromatography.
Immunoprecipitation and western blot analysis of extracts from mouse brain. Lane 1 contains lysate input, lane 2 was immunoprecipitated with Gα(o) Antibody (IP Preferred) and lane 3 was immunoprecipitated with non-specific rabbit IgG. Western blot analysis was performed using Gα(o) Antibody #3975.
Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).
Gα(o) is the most abundant G protein in the brain and couples to serotonin, dopamine, GABA (B), opioid, glutamate and cholinergic receptors (4). Gα(o) -/- mice have neurological defects such as tremors, seizures and poor motor coordination (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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