Cell Signaling Technology

Product Pathways - Neuroscience

GRK2 Antibody #3982

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Hm Mk Endogenous 80 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GRK2 Antibody detects endogenous levels of total GRK2. This antibody may also detect GRK3, but does not cross-react with other GRKs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human GRK2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos, THP1, U-937, and COS cells, using GRK2 Antibody.

IP

IP

Immunoprecipitation of GRK2 from Ramos, C6 and COS cell lysates followed by Western blot analysis, using GRK2 Antibody.

Background

G-protein-coupled receptor kinase 2 (GRK2), also known as beta-adrenergic receptor kinase 1 (beta-ARK1), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation (1). PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin (2,3). PKA phosphorylates GRK2 at Ser685, which facilitates the association of GRK2 with a beta-adrenergic receptor (4). Erk inhibits GRK2 activity via phosphorylation at Ser670 (5). Src phosphorylates GRK2 at multiple tyrosine residues (Tyr13, 86 and 92), which activates GRK2 activity and promotes GRK2 degradation (6,7).

  1. Pitcher, J. A. et al. (1998) Annu. Rev. Biochem. 67, 653-692.
  2. Winstel, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 2105-2109.
  3. Krasel, C. et al. (2001) J. Biol. Chem. 276, 1911-1915.
  4. Cong, M. et al. (2001) J. Biol. Chem. 276, 15192-15199.
  5. Pitcher, J. A. et al. (1999) J. Biol. Chem. 274, 34531-34534.
  6. Sarnago, S. et al. (1999) J. Biol. Chem. 274, 34411-34416.
  7. Penela, P. et al. (2001) EMBO J. 20, 5129-5138.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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