Product Pathways - Neuroscience
GAD2 Antibody #3988
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PhosphoSitePlus® protein, site, and accession data: GAD2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-F | M R (H) | Endogenous | 60 | Rabbit |
Applications Key:
W=Western Blotting
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
GAD2 Antibody detects endogenous levels of total GAD2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ala150 of human GAD2. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse and rat brain using GAD2 Antibody.
IF-F
Confocal immunofluorescent analysis of mouse retina using GAD2 Antibody (Red) and β3-Tubulin (TU-20) Mouse mAb #4466 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6).
- Kaufman, D.L. et al. (1991) J Neurochem 56, 720-3.
- Feldblum, S. et al. (1993) J Neurosci Res 34, 689-706.
- Gao, B. and Moore, R.Y. (1996) J Biol Rhythms 11, 172-9.
- Rubi, B. et al. (2001) J Biol Chem 276, 36391-6.
- Gilliam, L.K. et al. (2004) Clin Exp Immunol 138, 337-41.
- Skorstad, G. et al. (2008) Eur J Neurol 15, 973-80.
Application References
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Companion Products
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
