Product Pathways - Ca / cAMP / Lipid Signaling
Gα (pan) Antibody #3992
|3992S||100 µl (10 western blots)||---||In Stock||---|
|3992||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, Bovine||Endogenous||39, 41||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Gα (pan) Antibody detects total levels of endogenous G protein alpha subunits. The antibody may not detect G(12)-α, G(13)-α (13), G(s)-α (s) and G(olf)-α.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human guanine nucleotide-binding protein Gα (q) subunit. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from BAEC, HEK293, NIH/3T3, C6 and COS cells, using Gα (pan) Antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Gα (pan) Antibody in the presence of control peptide (left) or antigen specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Gα (pan) Antibody.
Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).
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