Cell Signaling Technology

Product Pathways - Neuroscience

Plexin A2 Antibody #3994

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 212 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Plexin A2 Antibody detects endogenous levels of total Plexin A2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Leu450 of human Plexin A2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected (lane 1) or transfected with a construct overexpressing Plexin A2 (lane 2), and mouse brain or rat brain using Plexin A2 Antibody.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).

Sema6A is also a ligand for Plexin A2. Both Sema6A and Plexin A2 knock-out mice have a granule cell migration defect, where cells remain in the molecular layer. Furthermore, Plexin A2 also controls nucleus-centrosome coupling which modulates cell migration (4).

  1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
  2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
  3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
  4. Renaud, J. et al. (2008) Nat Neurosci 11, 440-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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