Cell Signaling Technology

Product Pathways - Metabolism

IDH1 Antibody #3997

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 46 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IDH1 Antibody detects endogenous levels of total IDH1 protein. The antibody does not cross react with IDH2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the N terminus of human IDH1. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2 and NIH/3T3 cells using IDH1 Antibody.

Background

IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1). IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5).

  1. Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
  2. Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
  3. Hurley, J.H. et al. (1990) Science 249, 1012-6.
  4. Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
  5. Zhao, S. et al. (2009) Science 324, 261-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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