Product Pathways - Cytoskeletal Signaling
Phospho-Troponin I (Cardiac) (Ser23/24) Antibody #4004
PhosphoSitePlus® protein, site, and accession data: TNNI3
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 28 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4004:
- Western Blotting
Specificity / Sensitivity
Phospho-Troponin I (Cardiac) (Ser23/24) Antibody detects endogenous levels of cardiac troponin I only when phosphorylated at Ser23/24.This antibody does not cross-react with phosphorylated skeletal muscle troponin I.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser23/24 of human troponin I (cardiac). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse myocytes, untreated or stimulated with isoproterenol, using Phospho-Troponin I (Cardiac) (Ser23/24) Antibody (upper) or Troponin I Antibody #4002 (lower).
Background
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).
- Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
- Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
- Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.
Application References
- Birkeland, J.A. et al. (2007) Am J Physiol Heart Circ Physiol 293, H2367-76. Applications: Western Blotting
- Palpant, N.J. et al. (2008) Cardiovasc Res 80, 209-18. Applications: Western Blotting
- Charo, D.N. et al. (2009) Am J Physiol Heart Circ Physiol 297, H1904-13. Applications: Western Blotting
- De Arcangelis, V. et al. (2008) Mol Pharmacol 74, 1453-62. Applications: Western Blotting
- Haworth, R.S. et al. (2004) Circ Res 95, 1091-9. Applications: Western Blotting
- Sumandea, M.P. et al. (2008) J Biol Chem 283, 22680-9. Applications: Western Blotting
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Companion Products
- 4002 Troponin I Antibody
- 9621 Phospho-(Ser/Thr) PKA Substrate Antibody
- 2261 Phospho-(Ser) PKC Substrate Antibody
- 9921 Phospho-PKC Antibody Sampler Kit
- 9371 Phospho-PKC (pan) (βII Ser660) Antibody
- 9374 Phospho-PKCδ (Thr505) Antibody
- 9375 Phospho-PKCα/β II (Thr638/641) Antibody
- 9376 Phospho-PKCδ/θ (Ser643/676) Antibody
- 9379 Phospho-PKC (pan) (gamma Thr514) Antibody
- 2055 Phospho-PKCdelta (Tyr311) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
