Product Pathways - Metabolism
α-Amylase Antibody #4017
Have you tried your application using our XP® monoclonal antibodies? Try product: 3796
PhosphoSitePlus® protein, site, and accession data: AMY2B
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R (H) (M) | Endogenous | 58 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4017:
- Western Blotting
Specificity / Sensitivity
α-Amylase Antibody detects endogenous levels of total α-amylase protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human carcinoidα-amylase. Antibodies are purified by protein A and peptide affinity chromatography.
Background
α-amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). Studies reported the release of an ectopically expressed α-amylase in certain tumors (1). Furthermore, a new type of α-amylase (carcinoid α-amylase or AMY2B) was identified in a lung carcinoid tissue (2-4). The ectopic expression of α-amylase in a neuroendocrine tumor was also reported (5).
- Tomita, N. et al. (1988) Cancer Res 48, 3292-6.
- Tomita, N. et al. (1989) Gene 76, 11-8.
- Groot, P.C. et al. (1989) Gene 85, 567-8.
- Yokouchi, H. et al. (1990) Gene 90, 281-6.
- Daa, T. et al. (2002) J Exp Clin Cancer Res 21, 131-6.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2804 Hexokinase I Antibody
- 2867 Hexokinase II (C64G5) Rabbit mAb
- 2106 Hexokinase II Antibody
- 3198 PKM2 Antibody
- 3190 PKM1/2 (C103A3) Rabbit mAb
- 3186 PKM1/2 Antibody
- 3188 Aldolase A Antibody
- 2784 Pyruvate Dehydrogenase Antibody
- 3886 Glycogen Synthase (15B1) Rabbit mAb
- 3893 Glycogen Synthase Antibody
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.