Product Pathways - Neuroscience
Tau (Tau46) Mouse mAb #4019
|W IHC-P IF-P IF-F||H M R (B)||Endogenous||50 to 80||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Tau (Tau46) Mouse mAb detects endogenous levels of total tau protein and also cross-reacts with MAP2 at 280kD. Tau (Tau46) Mouse mAb is predicted to detect all six isoforms of tau based on the amino acid sequence.
Source / Purification
Monoclonal antibody is produced by immunizing animals with native bovine tau and the epitope maps to the carboxy-terminus of the protein.
Western blot analysis of extracts from mouse and rat brain, using Tau (Tau46) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using Tau (Tau46) Mouse mAb.
Confocal immunofluorescent analysis of mouse dentate gyrus stained with Tau (Tau46) Mouse mAb (red) and CREB (86B10) Mouse mAb #9104 (blue).
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
- Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
- Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
- Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
- Bramblett, G. T. et al. (1993) New phosphorylation sites identified in hyperphosphorylated tau (paired helical filament-tau) from Alzheimer's disease brain using nanoelectrospray mass spectrometry. Neuron 10, 1089-1099. Applications: IC-IF Western Blotting
- Kosik, K. S. et al. (1988) Epitopes that span the tau molecule are shared with paired helical filaments. Neuron 1, 817-825. Applications: IHC-P (paraffin) Western Blotting
- Horiguchi, T. et al. (2003) Nitration of tau protein is linked to neurodegeneration in tauopathies. Am. J. Pathol. 163, 1021-1031. Applications: Western Blotting
- Mawal-Dewan , M. et al. (1994) The phosphorylation state of tau in the developing rat brain is regulated by phosphoprotein phosphatases. J. Biol. Chem. 269, 30981-30987. Applications: Western Blotting
- Saiz-Sanchez, D. et al. (2010) Exp Neurol 223, 347-50. Applications: IF-P (paraffin)
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For Research Use Only. Not For Use In Diagnostic Procedures.