Product Pathways - Neuroscience

Tau (Tau46) Mouse mAb #4019

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Applications	Reactivity	MW (kDa)	Source	Isotype
W IP IHC-P IF-F IF-P	H M R (B)	50 to 80	Mouse	IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)  IF-P=Immunofluorescence (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Tau (Tau46) Mouse mAb detects endogenous levels of total tau protein and also cross-reacts with MAP2 at 280kD. Tau (Tau46) Mouse mAb is predicted to detect all six isoforms of tau based on the amino acid sequence.

Source / Purification

Monoclonal antibody is produced by immunizing mice with native bovine tau and the epitope maps to the carboxy-terminus of the protein.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

1. Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
2. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
3. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.

Application References

• Bramblett, G. T. et al. (1993) New phosphorylation sites identified in hyperphosphorylated tau (paired helical filament-tau) from Alzheimer's disease brain using nanoelectrospray mass spectrometry. Neuron 10, 1089-1099. This article references the use of Tau (Tau46) Mouse mAb in the following applications: IC-IF Western Blotting
• Kosik, K. S. et al. (1988) Epitopes that span the tau molecule are shared with paired helical filaments. Neuron 1, 817-825. This article references the use of Tau (Tau46) Mouse mAb in the following applications: IHC-P (paraffin) Western Blotting
• Horiguchi, T. et al. (2003) Nitration of tau protein is linked to neurodegeneration in tauopathies. Am. J. Pathol. 163, 1021-1031. This article references the use of Tau (Tau46) Mouse mAb in the following applications: Western Blotting
• Mawal-Dewan , M. et al. (1994) The phosphorylation state of tau in the developing rat brain is regulated by phosphoprotein phosphatases. J. Biol. Chem. 269, 30981-30987. This article references the use of Tau (Tau46) Mouse mAb in the following applications: Western Blotting

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