Product Pathways - Protein Folding/Stability
MMP-2 Antibody #4022
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 64, 72 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 4022:
- Western Blotting
Specificity / Sensitivity
MMP-2 Antibody detects full length (proenzyme, 72 kDa) and cleaved (active enzyme, 64 kDa) MMP-2.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues between 107-117 of human MMP-2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of partially purified, concentrated, serum-free medium containing human MMP-2, using MMP-2 Antibody.
Background
The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP2, MMP3, MMP7 and MMP9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).
- McCawley, L.J. and Matrisian, L.M. (2001) Curr. Opin. Cell Biol. 13, 534-540.
- Coussens, L.M. et al. (2002) Science 295, 2387-2391.
- Sternlicht, M.D. et al. (1999) Cell 98, 137-146.
- Vu, T.H. et al. (1998) Cell 93, 411-422.
- Nagase, H. et al. (1990) Biochemistry 29, 5783-5789.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
