Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Phospho-SATB1 (Ser47) Antibody #4028

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) (B) (Hr) Endogenous 100 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-SATB1 (Ser47) Antibody detects endogenous levels of SATB1 protein only when phosphorylated on Ser47.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser47 of the human SATB1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and THP-1 cell lines using Phospho-SATB1 (Ser47) Antibody (upper) or SATB1 (L745) Antibody #3650 (lower). Antibody phospho-specificity was determined by treating cell extracts with λ phosphatase.

Background

Special AT-rich binding protein 1 (SATB1) functions as both a global chromatin organizer and a gene-specific transcription factor (1). SATB1 cooperates with promyelocytic leukemia protein (PML) to regulate global chromatin architecture by organizing chromatin into distinct loops via periodic anchoring of matrix attachment regions (MARs) in DNA to the nuclear matrix (1-3). In addition, SATB1 recruits multiple chromatin-remodeling proteins that contribute to specific gene activation and repression, including the chromatin remodeling enzymes ACF and ISWI, the histone deacetylase HDAC1, and the histone acetyltransferases PCAF and p300/CBP (4-6). Phosphorylation of SATB1 on Ser185 by protein kinase C regulates its interaction with HDAC1 and PCAF. While unphosphorylated SATB1 binds to PCAF, phosphorylated SATB1 preferentially binds to HDAC1 (6). Acetylation of SATB1 on Lys136 by PCAF impairs its DNA binding activity, thereby removing SATB1 from gene promoters (6). SATB1 is expressed predominantly in thymocytes and is involved in gene regulation during T cell activation (1). SATB1 is also expressed in metastatic breast cancer cells and is a potential prognostic marker and therapeutic target for metastatic breast cancer (7). In a mouse model system, RNAi-mediated knockdown of SATB1 reversed tumorigenesis by inhibiting tumor growth and metastasis, while ectopic expression of SATB1 in non-metastatic breast cancer cells produced invasive tumors.

Phospho-SATB1 (Ser47) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser47 was discovered using an Akt substrate antibody. The function of this phosphorylation event is not known. Please visit PhosphoSitePlus™, CST's modification site knowledgebase, at www.phosphosite.org for more information.

  1. Galande, S. et al. (2007) Curr Opin Genet Dev 17, 408-14.
  2. Cai, S. et al. (2006) Nat Genet 38, 1278-88.
  3. Kumar, P.P. et al. (2007) Nat Cell Biol 9, 45-56.
  4. Yasui, D. et al. (2002) Nature 419, 641-5.
  5. Kumar, P.P. et al. (2005) Mol Cell Biol 25, 1620-33.
  6. Pavan Kumar, P. et al. (2006) Mol Cell 22, 231-43.
  7. Han, H.J. et al. (2008) Nature 452, 187-93.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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