Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

ROCK1 (C8F7) Rabbit mAb #4035

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 160 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ROCK1 (C8F7) Rabbit mAb detects endogenous levels of total ROCK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human ROCK1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ROCK1 (C8F7) Rabbit mAb.

IP

IP

Immunoprecipitation of ROCK1 from HeLa cell extracts using ROCK1 (C8F7) Rabbit mAb. Western blot detection was perfomed using the same antibody. Lane 1 is 5% input.

Background

ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6).

  1. Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
  2. Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
  3. Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471.
  4. Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249.
  5. Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260.
  6. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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