Product Pathways - Cytoskeletal Signaling
ROCK1 (C8F7) Rabbit mAb #4035
PhosphoSitePlus® protein, site, and accession data: ROCK1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 160 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
ROCK1 (C8F7) Rabbit mAb detects endogenous levels of total ROCK1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human ROCK1.
Western Blotting
Western blot analysis of extracts from various cell types using ROCK1 (C8F7) Rabbit mAb.
IP
Immunoprecipitation of ROCK1 from HeLa cell extracts using ROCK1 (C8F7) Rabbit mAb. Western blot detection was perfomed using the same antibody. Lane 1 is 5% input.
Background
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6).
- Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
- Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
- Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471.
- Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249.
- Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260.
- Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
Application References
- Mayanagi, T. et al. (2008) J Biol Chem 283, 31183-96. Applications: Western Blotting
- Park, J.H. et al. (2011) J Biol Chem 286, 23132-41. Applications: Western Blotting
- Hudson, C.A. et al. (2012) Mol Hum Reprod 18, 265-79. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
