Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) #4036

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 70 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) detects endogenous levels of ATF-2 only when phosphorylated at Thr71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or UV-treated, using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) and developed with Streptavidin-HRP #3999.

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112 reacts with human, mouse, rat and monkey phospho-ATF-2 protein. CST expects that Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) will also recognize phospho-ATF-2 in these species.

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

  1. Abdel-Hafiz, H.A. et al. (1992) Mol. Endocrinol. 6, 2079-2089.
  2. Gupta, S. et al. (1995) Science 267, 389-393.
  3. van Dam, H. et al. (1995) EMBO J. 14, 1798-1811.
  4. Livingstone, C. et al. (1995) EMBO J. 14, 1785-1797.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products