Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Jak/Stat Pathway

Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) #4040

Applications Reactivity Sensitivity MW (kDa) Isotype
W F H M R Endogenous 125 Rabbit IgG

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro841 of Jak2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of UT-7 cells using Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096 (red).

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same cross-reactivity as the unconjugated antibody (Jak2 (D2E12) XP® Rabbit mAb #3230).

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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