Product Pathways - Jak/Stat Pathway
Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) #4040
|4040S||100 µl (10 western blots)||---||In Stock||---|
|4040||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||125||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: W=Western Blotting, F=Flow Cytometry
Specificity / Sensitivity
Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro841 of Jak2 protein.
Western blot analysis of extracts from various cell lines using Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same cross-reactivity as the unconjugated antibody (Jak2 (D2E12) XP® Rabbit mAb #3230).
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.