Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

TCL1 Antibody #4042

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H (M) Endogenous 14 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TCL1 Antibody detects endogenous levels of total TCL1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human TCL1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Daudi and Ramos cells using TCL1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using TCL1 Antibody, low magnification (upper) and high magnification (lower).

Background

TCL1 (T cell leukemia 1), MTCP1 and TCL1b belong to the TCL1 proto-oncogene family, and their products are involved in Akt activation during embryonic development, T cell leukemias, prolymphocytic leukemias and B cell lymphomas (1-3). The Akt association domain of TCL1 binds with the PH domain of Akt. The formation of an oligomeric TCL-Akt complex is required for TCL1 coactivator function and results in phosphorylation and activation of Akt . Furthermore, functional analysis indicates that the interaction between TCL1 and Akt promotes translocation of Akt to the nucleus (4-6). These findings are supported by the crystal structure of TCL1, which suggests that TCL1 may participate in molecular transport (7).

  1. Narducci, M.G. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11712-11717.
  2. Pekarsky, P. et al. (2001) Oncogene 20, 5638-5643.
  3. Hoyer, K.K. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 14392-14397.
  4. Laine, J. et al. (2000) Mol. Cell 6, 395-407.
  5. Laine, J. et al. (2002) J. Biol. Chem. 277, 3743-3751.
  6. KŸnstle, G. et al. (2002) Mol. Cell. Biol. 22, 1513-1525.
  7. Petock, J.M. et al. (2002) Scientific World Journal 2, 1876-1884.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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