Cell Signaling Technology

Product Pathways - Protein Stability

HEF1/NEDD9 (2G9) Mouse mAb #4044

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 105, 115 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HEF1/NEDD9 (2G9) Mouse mAb detects endogenous levels of total HEF1/NEDD9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to residues from the human HEF1/NEDD9 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 and C2C12 cell lines using HEF1/NEDD9 (2G9) Mouse mAb.

Background

Human enhancer of filamentation protein 1 (HEF1), also known as neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), is part of the Cas family of proteins, which include HEF1/NEDD9, p130Cas and Efs (1). HEF1 is a predominantly cytoplasmic protein, localizing to focal adhesions during interphase, and centrosomes and other parts of the mitotic apparatus during G2/M phase of the cell cycle (2). HEF1 is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion, motility, growth and apoptosis (1). Phosphorylation of HEF1 is induced by a number of factors, including FAK, TGF-β, PDGFR, Abl, and BCR-ABL, which leads to coordinate binding of multiple downstream effector proteins via 15 known SH2 domain-binding sites (1). HEF1 is a key regulator of cancer metastasis. It is required for the invasive activity of glioblastomas (3), is linked to the promotion of metastasis in melanoma (4), and is found to be up-regulated in lung cancer metastasis (5).

  1. O'Neill, G.M. et al. (2007) Cancer Res 67, 8975-9.
  2. Law, S.F. et al. (1998) Mol Cell Biol 18, 3540-51.
  3. Natarajan, M. et al. (2006) Oncogene 25, 1721-32.
  4. Kim, M. et al. (2006) Cell 125, 1269-81.
  5. Ji, H. et al. (2007) Nature 448, 807-10.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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