Cell Signaling Technology

Product Pathways - Apoptosis

WWOX Antibody #4045

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) Endogenous 46 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WWOX Antibody detects endogenous levels of total WWOX protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr103 of WWOX. Antibodies were purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with mouse WWOX, and from MCF-7 and rat brain using WWOX Antibody.

Background

The WWOX (WW domain-containing oxidoreductase) gene encodes a protein with two WW domains followed by a short-chain dehydrogenase domain that was identified from a genomic region 16q23 of high instability, FRA16D (1,2). The mouse homolog, termed Wox1, was found to enhance TNFα-mediated apoptosis (3). The WWOX gene is disrupted in a many cancer types by deletions or translocation which has revealed a tumor suppressor function (4-7). In contrast, high levels of WWOX have been shown in shown in premetastic cancers, including breast and prostate (8-10). Stress stimuli can induce tyrosine phosphorylation within the first WW domain (Tyr33), followed by nuclear translocation and binding to and stabilizing the p53 tumor suppressor protein (11). WWOX and p53 can induce apoptosis in a synergistic manner. Tyrosine phosphorylation and nuclear translocation of WWOX has been implicated in the progression of cancers to metastatic states (10).

  1. Bednarek, A.K. et al. (2000) Cancer Res. 60, 2140-2145.
  2. Ried, K. et al. (2000) Hum. Mol. Genet. 9, 1651-1663.
  3. Chang, N.S. et al. (2001) J. Biol. Chem. 276, 3361-3370.
  4. Ramos, D. and Aldaz, C.M. (2006) Adv. Exp. Med. Biol. 587, 149-159.
  5. Paige, A.J. et al. (2001) Proc. Nat.l Acad. Sci. USA 98, 11417-11422.
  6. Bednarek, A.K. et al. (2001) Cancer Res. 61, 8068-8073.
  7. Aqeilan, R.I. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3949-3954.
  8. Driouch, K. et al. (2002) Oncogene 21, 1832-1840.
  9. Watanabe, A. et al. (2003) Cancer Res. 63, 8629-8633.
  10. Chang, N.S. et al. (2005) Oncogene 24, 714-723.
  11. Chang, N.S. et al. (2005) J. Biol. Chem. 280, 43100-43108.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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