Product Pathways - Glucose Metabolism
ACSL1 Antibody #4047
PhosphoSitePlus® protein, site, and accession data: ACSL1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R | Endogenous | 78 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4047:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
ACSL1 Antibody detects endogenous levels of total ACSL1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human ACSL1. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using ACSL1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ACSL1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ACSL1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using ACSL1 Antibody.
Background
Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (1). Five isoforms of ACSL have been identified (1). These isoforms have different substrate preferences and subcellular localizations (1). Overexpression of ACSL1 results in changes to fatty acid metabolism in rat primary hepatocytes (2).
- Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
- Li, L.O. et al. (2006) J Biol Chem 281, 37246-55.
- Li, L.O. et al. (2009) J Biol Chem , .
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
