Cell Signaling Technology

Product Pathways - Metabolism

ACSL1 Antibody #4047

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H M R Endogenous 78 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ACSL1 Antibody detects endogenous levels of total ACSL1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human ACSL1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ACSL1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ACSL1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ACSL1 Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using ACSL1 Antibody.

Background

Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (1). Five isoforms of ACSL have been identified (1). These isoforms have different substrate preferences and subcellular localizations (1). Overexpression of ACSL1 results in changes to fatty acid metabolism in rat primary hepatocytes (2).

  1. Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
  2. Li, L.O. et al. (2006) J Biol Chem 281, 37246-55.
  3. Li, L.O. et al. (2009) J Biol Chem , .

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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