Product Pathways - Translational Control
THEX1 Antibody #4049
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | M R | Endogenous | 40 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
THEX1 Antibody detects endogenous levels of total THEX1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of mouse THEX1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell types using THEX1 Antibody.
Background
THEX1 (3’hExo) is a 3’ exonuclease that may play a role in the degradation of histone mRNA transcripts (1). A recently identified member of the DEDDh 3' exonuclease family, THEX1, binds the conserved stem-loop structure found at the 3’ end of mRNA in vitro (2). The binding of THEX1 to mRNA requires the presence of a terminal ACCCA sequence and is enhanced by the concurrent binding of stem-loop binding protein (SLBP). Cleavage of histone mRNA by THEX1 exonuclease may help produce the rapid turnover of histone mRNA transcripts associated with the completion of DNA replication (3). Additional evidence suggests that THEX1 may be responsible for excising the remaining few 3’ nucleotides following cleavage by a different enzyme (4).
- Dominski, Z. and Marzluff, W.F. (1999) Gene 239, 1-14.
- Dominski, Z. et al. (2003) Mol Cell 12, 295-305.
- Yang, X.C. et al. (2006) J Biol Chem 281, 30447-54.
- Mullen, T.E. and Marzluff, W.F. (2008) Genes Dev 22, 50-65.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
