Cell Signaling Technology

Product Pathways - Translational Control

THEX1 (D66A11) Rabbit mAb #4055

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 46 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

THEX1 (D66A11) Rabbit mAb detects endogenous levels of total THEX1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Pro33 of human THEX1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using THEX1 (D66A11) Rabbit mAb.

Background

THEX1 (3’hExo) is a 3’ exonuclease that may play a role in the degradation of histone mRNA transcripts (1). A recently identified member of the DEDDh 3' exonuclease family, THEX1 binds the conserved stem-loop structure found at the 3’ end of mRNA in vitro (2). The binding of THEX1 to mRNA requires the presence of a terminal ACCCA sequence and is enhanced by the concurrent binding of stem-loop binding protein (SLBP). Cleavage of histone mRNA by THEX1 exonuclease may help produce the rapid turnover of histone mRNA transcripts associated with the completion of DNA replication (3). Additional evidence suggests that THEX1 may be responsible for excising the remaining few 3’ nucleotides following cleavage by a different enzyme (4).

  1. Dominski, Z. and Marzluff, W.F. (1999) Gene 239, 1-14.
  2. Dominski, Z. et al. (2003) Mol Cell 12, 295-305.
  3. Yang, X.C. et al. (2006) J Biol Chem 281, 30447-54.
  4. Mullen, T.E. and Marzluff, W.F. (2008) Genes Dev 22, 50-65.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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