Product Pathways - Adhesion

E-Cadherin Antibody #4065

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP IHC-P IF-IC	H M (B) (Dg)	Endogenous	135	Rabbit

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse B=Bovine Dg=Dog
Species cross-reactivity is determined by Western blot.

Protocols

4065:: IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

E-Cadherin Antibody detects endogenous levels of total E-Cadherin protein. The antibody does not cross-react with related family members, such as N-Cadherin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding residue 780 of human E-Cadherin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from A431, MCF-7 and C2C12 cells, using E-Cadherin Antibody. Note the absence of signal from C2C12 cells, which express N-cadherin instead of E-cadherin.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MCF-7 cells, showing membrane localization, using E-Cadherin Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using E-Cadherin Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung, showing membrane localization, using E-Cadherin Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast fibroadenoma, using E-Cadherin Antibody in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1056 (right).

IF-IC

Confocal immunofluorescent images of MCF-7 cells labeled with E-Cadherin Antibody (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).See notes for additional testing details.

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members which are found in adherens junctions (AJ), a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin) and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike β- and γ-catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-Cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression and localization are correlated with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

Application References

Chen, Y. et al. (2007) Mol Cell Proteomics 6, 2072-87. Applications: IP Western Blotting
Shan, B. et al. (2008) J Biol Chem 283, 21065-73. Applications: Western Blotting
Dougherty, S.M. et al. (2006) Endocr Relat Cancer 13, 113-34. Applications: Western Blotting
Wang, J. et al. (2009) J Histochem Cytochem 57, 363-71. Applications: IHC-P (paraffin)

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.