Cell Signaling Technology

Product Pathways - MAPK Signaling

Erk3 Antibody #4067

Applications Reactivity Sensitivity MW (kDa) Source
W IP F H M R Mk Endogenous 105 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Erk3 Antibody detects endogenous levels of total Erk3 protein. The antibody does not cross-react with other other Erk family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu410 of human Erk3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431, MCF7, HeLa, C6, NIH/3T3 and C2C12 cells, using Erk3 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated A431 cells, using Erk3 Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Erk3, also known as MAPK6 or p97 MAPK, is almost 50% identical to Erk1/2 at the kinase domain located in its amino-terminal region (1). However, Erk3 is distinguished from other MAP kinases in that it lacks the conserved TXY motif in its activation loop, possessing instead an SEG motif (1,2). Phosphorylation at Ser189 in the SEG motif has been reported (2,3). With limited information about its upstream kinases and downstream substrates, the significance of this phosphorylation remains to be elucidated (3,4). Erk3 is an inherently unstable protein, rapidly degraded through amino-terminal ubiquitination and proteasome degradation (3,5). A site-specific cleavage, depending on a short stretch of acidic residues of Erk3, might regulate its translocation from the Golgi/ERGIC to the nucleus during the cell cycle (6). Accumulating evidence suggests that Erk3 is involved in cell differentiation (1,3,6).

  1. Boulton, T. G. et al. (1991) Cell 65, 663-675.
  2. Cheng, M. et al. (1996) J. Biol. Chem. 271, 12057-12062.
  3. Coulombe, P. et al. (2003) Mol. Cell. Biol. 23, 4542-4558.
  4. Julien, C. et al. (2003) J. Biol. Chem. 278, 42615-42624.
  5. Coulombe, P. et al. (2004) Mol. Cell. Biol. 24, 6140-6150.
  6. Bind, E. et al. (2004) Mol. Biol. Cell 15, 4457-4466.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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