Product Pathways - MAPK Signaling
Erk3 Antibody #4067
|W IP F||H M R Mk||Endogenous||105||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Erk3 Antibody detects endogenous levels of total Erk3 protein. The antibody does not cross-react with other other Erk family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu410 of human Erk3. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from A431, MCF7, HeLa, C6, NIH/3T3 and C2C12 cells, using Erk3 Antibody.
Erk3, also known as MAPK6 or p97 MAPK, is almost 50% identical to Erk1/2 at the kinase domain located in its amino-terminal region (1). However, Erk3 is distinguished from other MAP kinases in that it lacks the conserved TXY motif in its activation loop, possessing instead an SEG motif (1,2). Phosphorylation at Ser189 in the SEG motif has been reported (2,3). With limited information about its upstream kinases and downstream substrates, the significance of this phosphorylation remains to be elucidated (3,4). Erk3 is an inherently unstable protein, rapidly degraded through amino-terminal ubiquitination and proteasome degradation (3,5). A site-specific cleavage, depending on a short stretch of acidic residues of Erk3, might regulate its translocation from the Golgi/ERGIC to the nucleus during the cell cycle (6). Accumulating evidence suggests that Erk3 is involved in cell differentiation (1,3,6).
- Boulton, T. G. et al. (1991) Cell 65, 663-675.
- Cheng, M. et al. (1996) J. Biol. Chem. 271, 12057-12062.
- Coulombe, P. et al. (2003) Mol. Cell. Biol. 23, 4542-4558.
- Julien, C. et al. (2003) J. Biol. Chem. 278, 42615-42624.
- Coulombe, P. et al. (2004) Mol. Cell. Biol. 24, 6140-6150.
- Bind, E. et al. (2004) Mol. Biol. Cell 15, 4457-4466.
- Hoeflich, K.P. et al. (2006) Int J Oncol 29, 839-49. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.