Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

GEF-H1 (55B6) Rabbit mAb #4076

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Hm Mk Endogenous 120 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GEF-H1 (55B6) Rabbit mAb detects endogenous levels of total GEF-H1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GEF-H1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types, using GEF-H1 (55B6) Rabbit mAb.

IP

IP

Immunoprecipitation of GEF-H1 from HeLa cell extracts, using GEF-H1 (55B6) Rabbit mAb. Western blot was performed using the same antibody.

Background

Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). PAK1 phosphorylates GEF-H1 at Ser885, a site located in the 14-3-3 binding motif. Phosphorylation at this site is required for 14-3-3 binding and correlated to recruitment of 14-3-3 and GEF-H1 to microtubules (3).GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability (4,5). GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest (5).

  1. Ren, Y. et al. (1998) J. Biol. Chem. 273, 34954-34960.
  2. Krendel, M. et al. (2002) Nat. Cell Biol. 4, 294-301.
  3. Zenke, F. T. et al. (2004) J. Biol. Chem. 279, 18392-18400.
  4. Benais-Pont, G. et al. (2003) J. Cell Biol. 160, 729-740.
  5. Aijaz, S. et al. (2005) Dev. Cell 8, 777-786.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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