Product Pathways - Cytoskeletal Signaling
GEF-H1 (55B6) Rabbit mAb #4076
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk Hm | Endogenous | 120 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Hm=Hamster
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
GEF-H1 (55B6) Rabbit mAb detects endogenous levels of total GEF-H1 protein.
Source / Purification
Monoclonal antibodies are produced by immunizing raabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence of human GEF-H1.
Western Blotting
Western blot analysis of extracts from various cell types, using GEF-H1 (55B6) Rabbit mAb.
IP
Immunoprecipitation of GEF-H1 from HeLa cell extracts, using GEF-H1 (55B6) Rabbit mAb. Western blot was performed using the same antibody.
Background
Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). PAK1 phosphorylates GEF-H1 at Ser885, a site located in the 14-3-3 binding motif. Phosphorylation at this site is required for 14-3-3 binding and correlated to recruitment of 14-3-3 and GEF-H1 to microtubules (3).GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability (4,5). GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest (5).
- Ren, Y. et al. (1998) J. Biol. Chem. 273, 34954-34960.
- Krendel, M. et al. (2002) Nat. Cell Biol. 4, 294-301.
- Zenke, F. T. et al. (2004) J. Biol. Chem. 279, 18392-18400.
- Benais-Pont, G. et al. (2003) J. Cell Biol. 160, 729-740.
- Aijaz, S. et al. (2005) Dev. Cell 8, 777-786.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
