Product Pathways - Companion Products
|4083S||1 mg||---||In Stock||---|
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DAPI is supplied as a lyophilized powder in 1 mg units. It can be used to examine cellular DNA in fluorescent microscopy applications.
4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).
Immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green), β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red) and DAPI (blue).
Directions For Use
This product is soluble up to 20 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, DAPI stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 to 0.1 μg/mL.
- Gannon, O.M. et al. (2013) Clin Cancer Res 19, 428-41. Applications: Companion Products.
- Lo, B. et al. (2012) J Cell Biol 199, 1117-30. Applications: Companion Products.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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