Cellular Dyes
DAPI #4083
Directions for Use
This product is soluble up to 20 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, DAPI stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 to 0.1 μg/mL.
IF-IC
Immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green), β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red) and DAPI (blue).
IF-IC
Immunofluorescent analysis of HUVE cells using VE-Cadherin (D87F2) XP® Rabbit mAb #2500 (green) and DAPI (blue). Actin filaments were labeled with DY-554 phalloidin (red).
Description
DAPI is supplied as a lyophilized powder in 1 mg units. It can be used to examine cellular DNA in fluorescent microscopy applications.
Background
4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).
- Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68.
- Kapuscinski, J. (1995) Biotech Histochem 70, 220-33.
- Manzini, G. et al. (1983) Nucleic Acids Res 11, 8861-76.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
