Directions for Use
This product is soluble up to 20 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, DAPI stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 to 0.1 μg/mL.
Immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green), β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red) and DAPI (blue).
DAPI is supplied as a lyophilized powder in 1 mg units. It can be used to examine cellular DNA in fluorescent microscopy applications.
4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).
- Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68.
- Kapuscinski, J. (1995) Biotech Histochem 70, 220-33.
- Manzini, G. et al. (1983) Nucleic Acids Res 11, 8861-76.
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For Research Use Only. Not For Use In Diagnostic Procedures.