Cellular Dyes
DRAQ5® #4084
Protocols
- 4084:
- Flow, Immunofluorescence
Directions for Use
Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:
Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.
Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 versus DRAQ5® (DNA content).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of mouse testis using Miwi (G82) Antibody #2079 (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
IF-P
Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Spectra
Absorption and fluorescence emission spectra of DRAQ5® in aqueous solution.
Background
DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).
- Smith, P.J. et al. (2000) Cytometry 40, 280-91.
- Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
- Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.
Application References
- Galitovskiy, V. et al. (2011) J Immunol 187, 2677-87. Applications: IF-IC (In Cells)
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Companion Products
DRAQ5® is a registered trademark of Biostatus Limited.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
